Overview
The Genome Sequencing Center at Washington University produces and distributes
DNA oligonucleotide microarrays representing the Candida albicans genome. The arrays are based on the recently published human-curated and annotated genome sequence of C. albicans strain SC5314 (Braun et. al., PLoS Genetics (2005)).
Design
The array represents 6,346 of the 6,354 predicted ORFs in the annotated C. albicans genome Assembly 19, and consists of one unique 70-mer oligonucleotide (chosen using ArrayOligoSelector) for each annotated open reading frame. Each oligonucleotide is designed to detect both alleles of the target gene. The array is printed 3 separate times per epoxy slide (19,038 spots total). Approximately 229 oligos (3.6%) may detect two separate ORFs, and this list is available here. The major repeat sequences (MRS) and the CTA2 gene family are not represented by unique oligos.
Controls
The array contains Stratagene's SpotReport® Oligo Array Validation System that includes 10 Arabidopsis control oligos, which are complimentary to Arabidopsis mRNA spikes available from Stratagene.
The price is $100 per slide. The pre-hybridization protocol and .gal files are available upon request. The Washington University Microarray Core Facility also offers probe preparation, hybridizations, and data analysis on a fee-for-service basis. For more information about pricing and services please contact the facility director, Seth Crosby, at scrosby@watson.wustl.edu.
Support
This project has been coordinated by Victoria Brown-Kennerly (vbrownk@genetics.wustl.edu), Seth Crosby (scrosby@watson.wustl.edu), Paul Cliften (cliften@biology.usu.edu), and Mark Johnston (mj@genetics.wustl.edu), and was supported by a grant from the Pfizer/Washington University Biomedical Research Program.
* Data generated with these arrays has been published in:
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