Eubacterium spp. are a group of anaerobic Gram-positive nonspore-forming rods. They have been poorly studied in terms of both physiology and genetics. No complete or partial genomes for members of this Genus are available. A complete, annotated genome sequence of a Eubacterium spp., whose predominance is comparable to Gram-negative gut inhabitants such as B. thetaiotaomicron, B. vulgatus and B. distasonis, should help reveal the extent to which abundantly represented Gram-negative and Gram-positive members of two distant Genera have evolved common and/or distinctive strategies to achieve symbiotic relationships with their human hosts. A16S rDNA-based enumeration study of bacteria in the feces of normal adult humans identified E. eligens and E. rectale as the centers of two clades [Operational Taxonomic Unit (OTU)]. E. eligens represents 9% of total sequenced amplicons [Appl Environ Microbiol. 65:4799 (1999)]. Therefore, it has been selected for complete genome sequencing.
Co-evolved symbiotic relationships between bacteria and multi-cellular organisms are a prominent feature of life on Earth. The ecosystem of human distal small intestine provides an opportunity to experimentally address general questions about symbiosis and ecogenomics. According to the most comprehensive 16S rDNA survey to-date [Science 308:1635 (2005)], this complex ecosystem is composed of ~400 phylotypes, the vast majority of which belong to two divisions of Bacteria: the Bacteroidetes (48%) and the Firmicutes (51%). Members of Eubacterium Genus are a predominant group among human intestinal Firmicutes.
The goal is to produce finished and annotated genome sequence from Eubacterium eligens type strain ATCC 27750. The McDonnell Genome Institute has collected 7X WGS coverage of plasmid end reads (21.5Mb Q20 bases, est. genome size 3Mb). Additional sequence coverage is being collected. Automated sequence improvement is currently ongoing. This project is funded by the National Science Foundation (NSF).