date set-up prep no agarose sequenced UP RUN Batch 1 RUN Batch 2 0 sequenced RP RUN Batch 1 RUN Batch 2 plate library vector received clone Project Funding date init date init grows gel comments clone date init date machine success date machine success attempts comment 0 clone date init date machine success date machine attempts comment success clone PNP0355 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20010618 rs71 Sommer 07-16-01 mg 07-18-01 rt 0 ok microwave boil prep/200ul ddH2O/-80 rs71 rs71 07-16-01/07-18-01 rt 30amda 176 63.00 -40RP 3700BDT .y1 PNP0355 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20010618 rs72 Sommer 07-16-01 mg 07-18-01 rt 0 ok microwave boil prep/200ul ddH2O/-80 rs72 rs72 07-16-01/07-18-01 rt 30amdb 176 84.00 -40RP 3700BDT .y1 PNP0355 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20010618 rs73 Sommer 07-16-01 mg 07-18-01 rt 0 ok microwave boil prep/200ul ddH2O/-80 rs73 rs73 07-16-01/07-18-01 rt 30amdc 176 81.00 -40RP 3700BDT .y1 PNP0355 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20010618 rs74 Sommer 07-16-01 mg 07-18-01 rt 2 ok microwave boil prep/200ul ddH2O/-80 rs74 rs74 07-16-01/07-18-01 rt 30amdd 176 87.00 -40RP 3700BDT .y1 PNP0356 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20010618 rs75 Sommer 07-16-01 mg 07-18-01 sk 0 ok microwave boil prep/200ul ddH2O/-80 rs75 rs75 07-18-01 sk 2amda 189 64 -40RP 3700BDT .y1 PNP0356 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20010618 rs76 Sommer 07-16-01 mg 07-18-01 sk 0 ok microwave boil prep/200ul ddH2O/-80 rs76 rs76 07-18-01 sk 2amdb 189 66 -40RP 3700BDT .y1 PNP0356 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20010618 rs77 Sommer 07-16-01 mg 07-18-01 sk 0 ok microwave boil prep/200ul ddH2O/-80 rs77 rs77 07-18-01 sk 2amdc 189 89 *h11 had no brew/-40RP 3700BDT .y1 PNP0356 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20010618 rs78 Sommer 07-16-01 mg 07-18-01 sk 0 ok microwave boil prep/200ul ddH2O/-80 rs78 rs78 07-18-01 sk 2amdd 189 76 -40RP 3700BDT .y1 PNP0357 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20010618 rs79 Sommer 07-11-01 mg 07-20-01 ir 0 variable *Set-up originally 7-11-01; test boxes failed; re-set up/microwave boil prep/200ul ddH2O/-80 rs79 rs79 07-23-01 ir 3amda 117 90.00 -40RP 3700BDT .y1 PNP0357 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20010618 rs80 Sommer 07-11-01 mg 07-20-01 ir 0 variable *Set-up originally 7-11-01; test boxes failed; re-set up/microwave boil prep/200ul ddH2O/-80 rs80 rs80 07-23-01 ir 3amdb 117 88.00 -40RP 3700BDT .y1 PNP0357 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20010618 rs81 Sommer 07-11-01 mg 07-20-01 ir 0 variable *Set-up originally 7-11-01; test boxes failed; re-set up/microwave boil prep/200ul ddH2O/-80 rs81 rs81 07-23-01 ir 3amdc 117 79.00 -40RP 3700BDT .y1 PNP0357 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20010618 rs82 Sommer 07-11-01 mg 07-20-01 ir 0 variable *Set-up originally 7-11-01; test boxes failed; re-set up/microwave boil prep/200ul ddH2O/-80 rs82 rs82 07-23-01 ir 3amdd 117 90.00 -40RP 3700BDT .y1 PNP0440 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs83 11-28-01 mg 12-05-01 jb 1 variable *pellets vary in size & color/microwave boil prep/360ul ddH2O/-80 rs83 rs83 12-06-01 jb 11amca 136 66.00 SL1 3700BDT .y1 PNP0440 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs84 11-28-01 mg 12-05-01 jb 0 variable *pellets vary in size & color/microwave boil prep/360ul ddH2O/-80 rs84 rs84 12-06-01 jb 11amcb 136 65.00 SL1 3700BDT .y1 PNP0440 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs85 11-28-01 mg 12-05-01 jb 1 variable *pellets vary in size & color/microwave boil prep/360ul ddH2O/-80 rs85 rs85 12-06-01 jb 11amcc 136 69.00 SL1 3700BDT .y1 PNP0440 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs86 11-28-01 mg 12-05-01 jb 2 variable *pellets vary in size & color/microwave boil prep/360ul ddH2O/-80 rs86 rs86 12-06-01 jb 11amcd 136 67.00 SL1 3700BDT .y1 PNP0441 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs87 11-28-01 mg 12-05-01 rt 2.00 variable microwave boil prep/300ul ddH2O/-80 rs87 rs87 12-04-01 rt 11amda 136 75.00 SL1 3700BDT .y1 PNP0441 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs88 11-28-01 mg 12-05-01 rt 1.00 variable microwave boil prep/300ul ddH2O/-80 rs88 rs88 12-04-01 rt 11amdb 136 84.00 SL1 3700BDT .y1 PNP0441 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs89 11-28-01 mg 12-05-01 rt 5.00 variable microwave boil prep/300ul ddH2O/-80 rs89 rs89 12-04-01 rt 11amdc 136 71.00 SL1 3700BDT .y1 PNP0441 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs90 11-28-01 mg 12-05-01 rt 1.00 variable microwave boil prep/300ul ddH2O/-80 rs90 rs90 12-04-01 rt 11amdd 136 76.00 SL1 3700BDT .y1 PNP0442 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs91 11-28-01 mg 12-05-01 lm 1 variable *pellets vary in size & color/microwave boil prep/360ul ddH2O/-80 rs91 rs91 12-06-01 lm 13amca 136 83.00 SL1 3700BDT .y1 PNP0442 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs92 11-28-01 mg 12-05-01 lm 0 variable *pellets vary in size & color/microwave boil prep/360ul ddH2O/-80 rs92 rs92 12-06-01 lm 13amcb 136 85.00 SL1 3700BDT .y1 PNP0442 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs93 11-28-01 mg 12-05-01 lm 0 variable *pellets vary in size & color/microwave boil prep/360ul ddH2O/-80 rs93 rs93 12-06-01 lm 13amcc 136 83.00 SL1 3700BDT .y1 PNP0442 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs94 11-28-01 mg 12-05-01 lm 4 variable *pellets vary in size & color/microwave boil prep/360ul ddH2O/-80 rs94 rs94 12-06-01 lm 13amcd 136 78.00 SL1 3700BDT .y1 PNP0443 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs95 12-12-01 mg 12-19-01 rt 0 variable microwave boil prep/360ul ddH2O/-80 rs95 rs95 12-17-01 rt 27amca 134 57.00 bad brew-re-seq as .y2 SL1 3700BDT .y1 PNP0443 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs96 12-12-01 mg 12-19-01 rt 0 variable microwave boil prep/360ul ddH2O/-80 rs96 rs96 12-17-01 rt 27amcb 134 56.00 bad brew-re-seq as .y2 SL1 3700BDT .y1 PNP0443 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs97 12-12-01 mg 12-19-01 rt 0 variable microwave boil prep/360ul ddH2O/-80 rs97 rs97 12-17-01 rt 27amcc 134 60.00 bad brew-re-seq as .y2 SL1 3700BDT .y1 PNP0443 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs98 12-12-01 mg 12-19-01 rt 0 variable microwave boil prep/360ul ddH2O/-80 rs98 rs98 12-17-01 rt 27amcd 134 79.00 bad brew-re-seq as .y2 SL1 3700BDT .y1 PNP0444 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rs99 12-12-01 mg 12-18-01 sk 0.00 variable microwave boil prep/360ul ddH2O/-80 rs99 rs99 12-17-01 sk 20amca 133 61.00 reseq'd as .y2 to check results by rt/bt SL1 3700BDT .y1 PNP0444 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rt01 12-12-01 mg 12-18-01 sk 2.00 variable microwave boil prep/360ul ddH2O/-80 rt01 rt01 12-17-01 sk 20amcb 133 73.00 reseq'd as .y2 to check results by rt/bt SL1 3700BDT .y1 PNP0444 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rt02 12-12-01 mg 12-18-01 sk 0.00 variable microwave boil prep/360ul ddH2O/-80 rt02 rt02 12-17-01 sk 20amcc 133 56.00 reseq'd as .y2 to check results by rt/bt SL1 3700BDT .y1 PNP0444 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rt03 12-12-01 mg 12-18-01 sk 1.00 variable microwave boil prep/360ul ddH2O/-80 rt03 rt03 12-17-01 sk 20amcd 133 62.00 reseq'd as .y2 to check results by rt/bt SL1 3700BDT .y1 PNP0445 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rt04 12-12-01 mg 12-18-01 er 2 variable microwave boil prep/360ul ddH2O/-80 rt04 rt04 12-17-01 er 20amda 133 42.00 reseq'd as .y2 to check results by rt/bt SL1 3700BDT .y1 PNP0445 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rt05 12-12-01 mg 12-18-01 er 0 variable microwave boil prep/360ul ddH2O/-80 rt05 rt05 12-17-01 er 20amdb 133 57.00 reseq'd as .y2 to check results by rt/bt SL1 3700BDT .y1 PNP0445 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rt06 12-12-01 mg 12-18-01 er 1 variable microwave boil prep/360ul ddH2O/-80 rt06 rt06 12-17-01 er 20amdc 133 56.00 reseq'd as .y2 to check results by rt/bt SL1 3700BDT .y1 PNP0445 Pristionchus_pacificus_mixed_stage_SL1_TOPO_v1_Murphy_Chiapelli_McCarter topo 20011119.00 rt07 12-12-01 mg 12-18-01 er 0 variable microwave boil prep/360ul ddH2O/-80 rt07 rt07 12-17-01 er 20amdd 133 45.00 reseq'd as .y2 to check results by rt/bt SL1 3700BDT .y1 PNP0358 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20020311.00 rt08 03-17-02 mg 03-20-02 jb 0 variable microwave boil prep/360ul ddH2O/-80 rt08 rt08 03-20-02 jb 22amca 112 84.00 -40RP 3700BDT .y1 PNP0358 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20020311.00 rt09 03-17-02 mg 03-20-02 jb 0 variable microwave boil prep/360ul ddH2O/-80 rt09 rt09 03-20-02 jb 22amcb 112 87.00 -40RP 3700BDT .y1 PNP0358 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20020311.00 rt10 03-17-02 mg 03-20-02 jb 0 variable microwave boil prep/360ul ddH2O/-80 rt10 rt10 03-20-02 jb 22amcc 112 90.00 -40RP 3700BDT .y1 PNP0358 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20020311.00 rt11 03-17-02 mg 03-20-02 jb 0 variable microwave boil prep/360ul ddH2O/-80 rt11 rt11 03-20-02 jb 22amcd 112 87.00 -40RP 3700BDT .y1 PNP0359 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20020311.00 rt12 03-17-02 mg 03-20-02 ir 0.00 variable microwave boil prep/200ul ddH2O/-80 rt12 rt12 03-19-02 ir 26amda 133 84.00 -40RP 3700BDT .y1 PNP0359 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20020311.00 rt13 03-17-02 mg 03-20-02 ir 0.00 variable microwave boil prep/200ul ddH2O/-80 rt13 rt13 03-19-02 ir 26amdb 133 80.00 -40RP 3700BDT .y1 PNP0359 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20020311.00 rt14 03-17-02 mg 03-20-02 ir 0.00 variable microwave boil prep/200ul ddH2O/-80 rt14 rt14 03-19-02 ir 26amdc 133 83.00 -40RP 3700BDT .y1 PNP0359 Prist_pacificus_mixed_stage_pAMP1_v1_Chiapelli_McCarter pAMP1 20020311.00 rt15 03-17-02 mg 03-20-02 ir 0.00 variable microwave boil prep/200ul ddH2O/-80 rt15 rt15 03-19-02 ir 26amdd 133 79.00 -40RP 3700BDT .y1