The Caenorhabditis sp.4 (PB2801) was sequenced to a depth of 9.5X
using plasmid and fosmid libraries made from DNA extracted for a 
mixed population of females and males from all developmental stages.  
Coverage was based on an expected genome size of 150 Mb.   The 
whole-genome shotgun plasmid and fosmid paired end reads were assembled 
using PCAP (Genome Res. 13(9):2164-70 2003). The assembly can be 
downloaded from the GSC ft site.  Additional plasmid and fosmid 
coverage and primer-directed sequence improvement are ongoing at 
the GSC.

For answers to questions about this assembly or project, or any 
other GSC genome project, please visit our Genome Groups web page 
(http://genome.wustl.edu/genome_group_index.cgi) 
and email the designated contact person.

Funding for the sequence characterization of this genome was provided 
by the National Human Genome Research Institute (NHGRI), National 
Institutes of Health (NIH).



*** SIMPLE READ STATS ***
Total input reads: 2800292
Total input bases: 2112410911 bp
Total Q20 bases: 1673926516 bp
Average Q20 bases per read: 598 bp
Average read length: 754 bp
Placed reads: 2707472
  (reads in scaffold: 2490418)
  (reads in singleton: 217054)
Unplaced reads: 92820
Chaff rate: 3.31%
Q20 base redundancy; 9.5X
Total prefin reads input: 0
Total prefin reads unused: 0


*** Contiguity: Contig ***
Total contig number: 29348
Total contig bases: 175247318 bp
Total Q20 bases: 172405520 bp
Q20 bases %: 98.3%
Average contig length: 5971 bp
Maximum contig length: 145414 bp
N50 contig length: 14709 bp
N50 contig number: 3065

Major contig (> 2000 bp) number: 15768
Major_contig bases: 159013477 bp
Major_contig Q20 bases: 157411729 bp
Major_contig Q20 base percent: 99%

Top tier (up to 45000000 bp): 
  Contig number: 979
  Average length: 45968 bp
  Longest length: 145414 bp
  Contig bases in this tier: 45002785 bp
  Q20 bases in this tier: 44888183 bp
  Q20 base percentage: 99.7%
  Top tier N50 contig length: 45881 bp
  Top tier N50 contig number: 356

Middle tier (45000000 bp -- 55000000 bp): 
  Contig number: 371
  Average length: 26996 bp
  Longest length: 29638 bp
  Contig bases in this tier: 10015360 bp
  Q20 bases in this tier: 9973813 bp
  Q20 base percentage: 99.5%
  Middle tier N50 contig length: 26960 bp
  Middle tier N50 contig number: 178

Bottom tier (55000000 bp -- end): 
  Contig number: 27998
  Average length: 4294 bp
  Longest length: 25024 bp
  Contig bases in this tier: 120229173 bp
  Q20 bases in this tier: 117543524 bp
  Q20 base percentage: 97.7%
  Bottom tier N50 contig length: 9027 bp
  Bottom tier N50 contig number: 4104



*** Contiguity: Supercontig ***
Total supercontig number: 10292
Average supercontig length: 17028 bp
Maximum supercontig length: 1093989 bp
N50 supercontig length: 88950 bp
N50 supercontig number: 441

Major supercontig (> 2000 bp) number: 5273
Major_supercontig bases: 169776609 bp
Major_supercontig Q20 bases: 167465397 bp
Major_supercontig Q20 base percent: 98.6%

Scaffolds > 1M: 2
Scaffold 250K--1M: 93
Scaffold 100K--250K: 275
Scaffold 10--100K: 2735
Scaffold 5--10K: 993
Scaffold 2--5K: 1174
Scaffold 0--2K: 5020

Top tier (up to 45000000 bp): 
  Supercontig number: 118
  Average length: 381848 bp
  Longest length: 1093989 bp
  Contig bases in this tier: 45058105 bp
  Q20 bases in this tier: 44694233 bp
  Q20 base percentage: 99.1%
  Top tier N50 supercontig length: 410485 bp
  Top tier N50 supercontig number: 39

Middle tier (45000000 bp -- 55000000 bp): 
  Supercontig number: 51
  Average length: 198119 bp
  Longest length: 228321 bp
  Contig bases in this tier: 10104049 bp
  Q20 bases in this tier: 10014339 bp
  Q20 base percentage: 99.1%
  Middle tier N50 supercontig length: 198598 bp
  Middle tier N50 supercontig number: 24

Bottom tier (55000000 bp -- end): 
  Supercontig number: 10123
  Average length: 11863 bp
  Longest length: 178785 bp
  Contig bases in this tier: 120085164 bp
  Q20 bases in this tier: 117696948 bp
  Q20 base percentage: 98%
  Bottom tier N50 supercontig length: 44660 bp
  Bottom tier N50 supercontig number: 708



*** Constraints ***
No. of satisfied constraints in contigs:      781851
No. of unsatisfied in distance in contigs:      4143
No. of satisfied links in scaffolds:          203406
No. of unsatisfied in dist. in scaffolds:       6268
No. of unsatisfied due to singlets:           178409
No. of unsatisfied due to short scaffolds:     38292
No. of unsatisfied due to scaffold ends:       14509
No. of other unsatisfied constraints:          32451
No. of redundant constraints:                    204
Total no. of satisfied constraints:           985257
Total no. of unsatisfied constraints:         274072
Total no. of constraints:                    1259533


*** Genome Contents ***
Total GC count: 67774859, (38.7%)
Total AT count: 107472459, (61.3%)
Total NX count: 0, (0%)
Total: 175247318

Total fosmids assembled in scaffold: 110162
Total length of these reads: 94849423
Paired reads: 90776 (82.4%)
Estimated fosmid coverage: .43X