The Caenorhabditis japonica genome was sequenced to a depth of 6.3X using plasmid and fosmid libraries made from DNA extracted from a mixed population of females and males from all developmental stages. The expected genome size is 83 Mb. The whole-genome shotgun plasmid and fosmid paired end reads were assembled using PCAP (Genome Res. 13(9):2164-70 2003). Additional coverage and primer-directed sequence improvement are ongoing at the GSC. Caenorhabditis japonica is described by Kiontke, Hironaka & Sudhaus (Nematology 4:933-941, 2002) and the strain sequence is DF5081. This strain was derived from the wild isolate DF5080. It was inbred by Karin Kiontke in the laboratory of David Fitch for 20 generations by transferring one young plugged gravid female per generation. It is likely that this female had mated with only one of her brothers. The strain DF5080 was established with 20 individuals collected from a dead shield bug Parastrachia japonensis in Takeo, Japan by Toyoshi Yoshiga. Takeo is 30 km away from Hinokuma Mountain Prefectural Park, the locus typicus of C. japonica. For answers to questions about this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. Funding for the sequence characterization of this genome was provided by the National Human Genome Research Institute (NHGRI), National Institutes of Health (NIH). *** Contiguity: Contig *** Total contig number: 35286 Total contig bases: 129372961 bp Total Q20 bases: 124576772 bp Q20 bases %: 96.2% Average contig length: 3666 bp Average major (> 2000 bp) contig length: 5501 Maximum contig length: 51942 bp N50 contig length: 5781 bp N50 contig number: 6367 Major contig (> 2000 bp) number: 20069 Major_contig bases: 110404923 bp Major_contig Q20 bases: 107512353 bp Major_contig Q20 base percent: 97.4% Top tier (up to 90000000 bp): Contig number: 12132 Average length: 7419 bp Longest length: 51942 bp Contig bases in this tier: 90002401 bp Q20 bases in this tier: 88256438 bp Q20 base percentage: 98% Top tier N50 contig length: 8258 bp Top tier N50 contig number: 3512 Middle tier (90000000 bp -- 120000000 bp): Contig number: 13581 Average length: 2209 bp Longest length: 3326 bp Contig bases in this tier: 30001122 bp Q20 bases in this tier: 28069618 bp Q20 base percentage: 93.5% Middle tier N50 contig length: 2313 bp Middle tier N50 contig number: 5421 Bottom tier (120000000 bp -- end): Contig number: 9573 Average length: 979 bp Longest length: 1418 bp Contig bases in this tier: 9369438 bp Q20 bases in this tier: 8250716 bp Q20 base percentage: 88% Bottom tier N50 contig length: 1093 bp Bottom tier N50 contig number: 3746 *** Contiguity: Supercontig *** Total supercontig number: 4670 Average supercontig length: 27703 bp Maximum supercontig length: 956079 bp N50 supercontig length: 62153 bp N50 supercontig number: 558 Major supercontig (> 2000 bp) number: 4454 Major_supercontig bases: 129009969 bp Major_supercontig Q20 bases: 124256166 bp Major_supercontig Q20 base percent: 96.3% Scaffolds > 1M: 0 Scaffold 250K--1M: 24 Scaffold 100K--250K: 230 Scaffold 10--100K: 2547 Scaffold 5--10K: 562 Scaffold 2--5K: 1091 Scaffold 0--2K: 216 Top tier (up to 90000000 bp): Supercontig number: 1114 Average length: 80796 bp Longest length: 956079 bp Contig bases in this tier: 90006526 bp Q20 bases in this tier: 87032180 bp Q20 base percentage: 96.6% Top tier N50 supercontig length: 93052 bp Top tier N50 supercontig number: 296 Middle tier (90000000 bp -- 120000000 bp): Supercontig number: 1555 Average length: 19298 bp Longest length: 32727 bp Contig bases in this tier: 30008049 bp Q20 bases in this tier: 28738176 bp Q20 base percentage: 95.7% Middle tier N50 supercontig length: 20886 bp Middle tier N50 supercontig number: 574 Bottom tier (120000000 bp -- end): Supercontig number: 2001 Average length: 4677 bp Longest length: 10921 bp Contig bases in this tier: 9358386 bp Q20 bases in this tier: 8806416 bp Q20 base percentage: 94.1% Bottom tier N50 supercontig length: 7431 bp Bottom tier N50 supercontig number: 502 *** SNP Stats *** Unable to find any *snp files to process stats