The Caenorhabditis remanei genome was sequenced to a depth of 9X using whole-genome shotgun plasmid and fosmid libraries made from DNA extracted from a mixture of larvae and adult males and females. Coverage was based on an expected genome size of 100 Mb. The whole-genome shotgun plasmid and fosmid paired end reads were assembled using PCAP (Genome Res. 13(9):2164-70 2003) and submitted to GenBank under the accession number AAGD00000000. The assembly can also be downloaded from the GSC ft site. Additional plasmid and fosmid coverage and primer-directed sequence improvement are ongoing at the GSC. A preliminary gene set is available on the WormBase Genome Browser (http://dev.wormbase.org/db/seq/gbrowse/remanei/). For answers to questions about this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. Funding for the sequence characterization of the Drosophila simulans genome was provided by the National Human Genome Research Institute (NHGRI), National Institutes of Health (NIH). Production Sequencing statistics: project: Caenorhabditis_remanei-1.0 ** SIMPLE READ STATS *** total input reads: 1561587 Total input bases: 1339466211 bp Total Q20 bases: 1088291339 bp Average Q20 bases per read: 697 bp Average read length: 858 bp Placed reads: 1472948 Unplaced reads: 88639 Chaff rate: 5.7% ** Contiguity: Contig *** Total contig number: 27989 Total contig bases: 146898439 bp Total Q20 bases: 142013601 bp Q20 bases %: 96.6% Average contig length: 5248 bp Maximum contig length: 219156 bp Major contig (> 2000 bp) number: 13353 N50 contig length: 13337 bp N50 contig number: 2505 *** Contiguity: Supercontig *** Total supercontig number: 9660 Average supercontig length: 15207 bp Maximum supercontig length: 5867231 bp Major supercontig (> 20000 bp) number: 495 N50 supercontig length: 392930 bp N50 supercontig number: 74 *** Constraints *** No. of satisfied constraints in contigs: 419034 No. of unsatisfied in distance in contigs: 2568 No. of satisfied links in scaffolds: 128715 No. of unsatisfied in dist. in scaffolds: 6631 No. of unsatisfied due to singlets: 158836 No. of unsatisfied due to short scaffolds: 30806 No. of unsatisfied due to scaffold ends: 1185 No. of other unsatisfied constraints: 18491 No. of redundant constraints: 13982 Total no. of satisfied constraints: 547749 Total no. of unsatisfied constraints: 218517 Total no. of constraints: 780248 *** Genome Contents *** Total GC count: 55841683, (38%) Total AT count: 91053892, (62%) Total NX count: 2864, (0%)