The strain of Enterobacter sakazakii being sequenced was isolated from powdered
milk formula fed to a hospitalized neonate that developed an infection.
http://www.cdc.gov/mmwr/preview/mmwrhtml/mm5114a1.html/  It is available from 
the American Type Culture Collection as ATCC BAA-894 or from the Salmonella 
Genetic Stock Centre (www.ucalgary.ca/~kesander) as SGSC4695.  This draft 
assembly is a 6.1X WGS of plasmid and fosmid end sequences.  There will be
automated sequence improvement (pre-finishing), and the genome will be finished
manually; therefore, several more assemblies are anticipated.  The combined 
sequence reads were assembled using the PCAP software 
(Genome Res. 13(9):2164-70 2003).  

For answers to your questions regarding this assembly or project, or any other
GSC genome project, please visit our Genome Groups web page 
(http://genome.wustl.edu/genomegroup_index.cgi) and email the designated contact 
person.

The National Institute of Allergy and Infectious Diseases (NIAID) is providing
funding for the sequence characterization of the Enterobacter sakazakii genome.

Production Statistics:

*** SIMPLE READ STATS ***
Total input reads: 46367
Total input bases: 36420022 bp
Total Q20 bases: 27442376 bp
Average Q20 bases per read: 592 bp
Average read length: 785 bp
Placed reads: 44642
Unplaced reads: 1725
Chaff rate: 3.72%
Q20 base redundancy: 6.1X
Total prefin reads input: 0
Total prefin reads unused: 0


*** Contiguity: Contig ***
Total contig number: 674
Total contig bases: 4435351 bp
Total Q20 bases: 4355020 bp
Q20 bases %: 98.1%
Average contig length: 6581 bp
Maximum contig length: 131657 bp
Major contig (> 1000 bp) number: 598
N50 contig length: 11961 bp
N50 contig number: 98

Top tier (up to 4000000 bp): 
  Contig number: 380
  Average length: 10532 bp
  Longest length: 131657 bp
  Contig bases in this tier: 4002340 bp
  Q20 bases in this tier: 3955337 bp
  Q20 base percentage: 98.8%
  Top tier N50 contig length: 13541 bp
  Top tier N50 contig number: 81

Middle tier (4000000 bp -- 4300000 bp): 
  Contig number: 155
  Average length: 1941 bp
  Longest length: 2961 bp
  Contig bases in this tier: 300878 bp
  Q20 bases in this tier: 281240 bp
  Q20 base percentage: 93.4%
  Middle tier N50 contig length: 2046 bp
  Middle tier N50 contig number: 62

Bottom tier (4300000 bp -- end): 
  Contig number: 139
  Average length: 951 bp
  Longest length: 1270 bp
  Contig bases in this tier: 132133 bp
  Q20 bases in this tier: 118443 bp
  Q20 base percentage: 89.6%
  Bottom tier N50 contig length: 1017 bp
  Bottom tier N50 contig number: 57



*** Contiguity: Supercontig ***
Total supercontig number: 133
Average supercontig length: 33349 bp
Maximum supercontig length: 903272 bp
Major supercontig (> 1000 bp) number: 84
N50 supercontig length: 748644 bp
N50 supercontig number: 3

Top tier (up to 4000000 bp): 
  Supercontig number: 6
  Average length: 683655 bp
  Longest length: 903272 bp
  Contig bases in this tier: 4101929 bp
  Q20 bases in this tier: 4037184 bp
  Q20 base percentage: 98.4%
  Top tier N50 supercontig length: 748644 bp
  Top tier N50 supercontig number: 3

Middle tier (4000000 bp -- 4300000 bp): 
  Supercontig number: 85
  Average length: 3535 bp
  Longest length: 131657 bp
  Contig bases in this tier: 300438 bp
  Q20 bases in this tier: 288461 bp
  Q20 base percentage: 96%
  Middle tier N50 supercontig length: 131657 bp
  Middle tier N50 supercontig number: 1

Bottom tier (4300000 bp -- end): 
  Supercontig number: 42
  Average length: 785 bp
  Longest length: 951 bp
  Contig bases in this tier: 32984 bp
  Q20 bases in this tier: 29375 bp
  Q20 base percentage: 89%
  Bottom tier N50 supercontig length: 951 bp
  Bottom tier N50 supercontig number: 1



*** Constraints ***
No. of satisfied constraints in contigs:       12404
No. of unsatisfied in distance in contigs:       766
No. of satisfied links in scaffolds:            3420
No. of unsatisfied in dist. in scaffolds:         97
No. of unsatisfied due to singlets:             2974
No. of unsatisfied due to short scaffolds:       330
No. of unsatisfied due to scaffold ends:           6
No. of other unsatisfied constraints:            212
No. of redundant constraints:                    130
Total no. of satisfied constraints:            15824
Total no. of unsatisfied constraints:           4385
Total no. of constraints:                      20339


*** Genome Contents ***
Total GC count: 2513724, (56.7%)
Total AT count: 1921627, (43.3%)
Total NX count: 0, (0%)
Total: 4435351


*** Fosmid Coverage ***
total fosmids used: 7217
total length of these fosmids: 6131648 bp
Estimated fosmid coverage: 1.04X