The strain of Enterobacter sakazakii being sequenced was isolated from 
powdered milk formula fed to a hospitalized neonate that developed an 
infection. http://www.cdc.gov/mmwr/preview/mmwrhtml/mm5114a1.html/  It
is available from the American Type Culture Collection as ATCC BAA-894
or from the Salmonella Genetic Stock Centre (www.ucalgary.ca/~kesander)
as SGSC4695.  This draft assembly is an 6.2X WGS of plasmid and fosmid
end sequences plus 1 round of automated sequence improvement 
(pre-finishing) reads.  The genome will be finished manually; therefore, 
several more assemblies are anticipated.  The combined sequence reads 
were assembled using the PCAP software (Genome Res. 13(9):2164-70 2003).  

For answers to your questions regarding this assembly or project, or any
other GSC genome project, please visit our Genome Groups web page 
(http://genome.wustl.edu/genomegroup_index.cgi) and email the designated
contact person.

The National Institute of Allergy and Infectious Diseases (NIAID),
National Institutes of Health (NIH) is providing funding for the sequence 
characterization of the Enterobacter sakazakii genome.

Production Sequencing Statistics:

*** SIMPLE READ STATS ***
Total input reads: 47812
Total input bases: 37623017 bp
Total Q20 bases: 28230380 bp
Average Q20 bases per read: 590 bp
Average read length: 787 bp
Placed reads: 46009
Unplaced reads: 1803
Chaff rate: 3.77%
Q20 base redundancy: 6.2X
Total prefin reads input: 1445
Total prefin reads unused: 135


*** Contiguity: Contig ***
Total contig number: 383
Total contig bases: 4515561 bp
Total Q20 bases: 4458511 bp
Q20 bases %: 98.7%
Average contig length: 11790 bp
Maximum contig length: 131657 bp
Major contig (> 1000 bp) number: 324
N50 contig length: 26773 bp
N50 contig number: 50

Top tier (up to 4000000 bp): 
  Contig number: 166
  Average length: 24129 bp
  Longest length: 131657 bp
  Contig bases in this tier: 4005406 bp
  Q20 bases in this tier: 3977665 bp
  Q20 base percentage: 99.3%
  Top tier N50 contig length: 30679 bp
  Top tier N50 contig number: 41

Middle tier (4000000 bp -- 4200000 bp): 
  Contig number: 32
  Average length: 6259 bp
  Longest length: 7387 bp
  Contig bases in this tier: 200300 bp
  Q20 bases in this tier: 193858 bp
  Q20 base percentage: 96.7%
  Middle tier N50 contig length: 6243 bp
  Middle tier N50 contig number: 15

Bottom tier (4200000 bp -- end): 
  Contig number: 185
  Average length: 1675 bp
  Longest length: 5555 bp
  Contig bases in this tier: 309855 bp
  Q20 bases in this tier: 286988 bp
  Q20 base percentage: 92.6%
  Bottom tier N50 contig length: 1841 bp
  Bottom tier N50 contig number: 43



*** Contiguity: Supercontig ***
Total supercontig number: 106
Average supercontig length: 42600 bp
Maximum supercontig length: 2556433 bp
Major supercontig (> 1000 bp) number: 62
N50 supercontig length: 2556433 bp
N50 supercontig number: 1

Top tier (up to 4000000 bp): 
  Supercontig number: 4
  Average length: 1060489 bp
  Longest length: 2556433 bp
  Contig bases in this tier: 4241957 bp
  Q20 bases in this tier: 4197653 bp
  Q20 base percentage: 98.9%
  Top tier N50 supercontig length: 2556433 bp
  Top tier N50 supercontig number: 1

Middle tier (4000000 bp -- 4200000 bp): 
  Supercontig number: 25
  Average length: 8015 bp
  Longest length: 131657 bp
  Contig bases in this tier: 200363 bp
  Q20 bases in this tier: 196266 bp
  Q20 base percentage: 97.9%
  Middle tier N50 supercontig length: 131657 bp
  Middle tier N50 supercontig number: 1

Bottom tier (4200000 bp -- end): 
  Supercontig number: 77
  Average length: 951 bp
  Longest length: 1334 bp
  Contig bases in this tier: 73241 bp
  Q20 bases in this tier: 64592 bp
  Q20 base percentage: 88.1%
  Bottom tier N50 supercontig length: 1334 bp
  Bottom tier N50 supercontig number: 1



*** Constraints ***
No. of satisfied constraints in contigs:       13852
No. of unsatisfied in distance in contigs:       788
No. of satisfied links in scaffolds:            2553
No. of unsatisfied in dist. in scaffolds:        129
No. of unsatisfied due to singlets:             3240
No. of unsatisfied due to short scaffolds:       315
No. of unsatisfied due to scaffold ends:          44
No. of other unsatisfied constraints:            975
No. of redundant constraints:                   1118
Total no. of satisfied constraints:            16405
Total no. of unsatisfied constraints:           5491
Total no. of constraints:                      23014


*** Genome Contents ***
Total GC count: 2560066, (56.7%)
Total AT count: 1955495, (43.3%)
Total NX count: 0, (0%)
Total: 4515561


*** Fosmid Coverage ***
total fosmids used: 8142
total length of these fosmids: 6921616 bp
Estimated fosmid coverage: 1.14X