The strain of Enterobacter sakazakii being sequenced was 
isolated from powdered milk formula fed to a hospitalized
neonate who developed an infection. 
http://www.cdc.gov/mmwr/preview/mmwrhtml/mm5114a1.html/  It
is available from the American Type Culture Collection as 
ATCC BAA-894 or from the Salmonella Genetic Stock Centre 
(www.ucalgary.ca/~kesander) as SGSC4695.  This draft assembly 
is an 6.4X WGS of plasmid and fosmid end sequences plus 2
rounds of automated sequence improvement (pre-finishing) 
reads.  The genome will be finished manually; therefore, 
several more assemblies are anticipated.  The combined 
sequence reads were assembled using the PCAP software (Genome 
Res. 13(9):2164-70 2003).  

For answers to your questions regarding this assembly or 
project, or any other GSC genome project, please visit our 
Genome Groups web page 
(http://genome.wustl.edu/genome_group_index.cgi) and email 
the designated contact person.

The National Institute of Allergy and Infectious Diseases 
(NIAID),National Institutes of Health (NIH) is providing 
funding for the sequence characterization of the Enterobacter 
sakazakii genome.

Production Sequencing Statistics:

*** SIMPLE READ STATS ***
Total input reads: 48525
Total input bases: 38185305 bp
Total Q20 bases: 28573089 bp
Average Q20 bases per read: 589 bp
Average read length: 787 bp
Placed reads: 46574
  (reads in scaffold: 42376)
Unplaced reads: 1951
Chaff rate: 4.02%
Q20 base redundancy: 6.4X
Total prefin reads input: 1957
Total prefin reads unused: 210


*** Contiguity: Contig ***
Total contig number: 312
Total contig bases: 4452184 bp
Total Q20 bases: 4405330 bp
Q20 bases %: 98.9%
Average contig length: 14270 bp
Maximum contig length: 131657 bp
N50 contig length: 42552 bp
N50 contig number: 32

Major contig (> 2000 bp) number: 161
Major_contig bases: 4280810 bp
Major_contig Q20 bases: 4251680 bp
Major_contig Q20 base percent: 99.3%

Top tier (up to 4000000 bp): 
  Contig number: 111
  Average length: 36069 bp
  Longest length: 131657 bp
  Contig bases in this tier: 4003642 bp
  Q20 bases in this tier: 3983010 bp
  Q20 base percentage: 99.4%
  Top tier N50 contig length: 50635 bp
  Top tier N50 contig number: 27

Middle tier (4000000 bp -- 4200000 bp): 
  Contig number: 27
  Average length: 7528 bp
  Longest length: 10243 bp
  Contig bases in this tier: 203267 bp
  Q20 bases in this tier: 198485 bp
  Q20 base percentage: 97.6%
  Middle tier N50 contig length: 7972 bp
  Middle tier N50 contig number: 11

Bottom tier (4200000 bp -- end): 
  Contig number: 174
  Average length: 1410 bp
  Longest length: 5430 bp
  Contig bases in this tier: 245275 bp
  Q20 bases in this tier: 223835 bp
  Q20 base percentage: 91.2%
  Bottom tier N50 contig length: 1559 bp
  Bottom tier N50 contig number: 50



*** Contiguity: Supercontig ***
Total supercontig number: 124
Average supercontig length: 35905 bp
Maximum supercontig length: 1214348 bp
N50 supercontig length: 660143 bp
N50 supercontig number: 3

Major supercontig (> 2000 bp) number: 21
Major_supercontig bases: 4340317 bp
Major_supercontig Q20 bases: 4306622 bp
Major_supercontig Q20 base percent: 99.2%

Scaffolds > 1M: 1
Scaffold 250K--1M: 5
Scaffold 100K--250K: 2
Scaffold 0--100K: 116

Top tier (up to 4000000 bp): 
  Supercontig number: 6
  Average length: 669466 bp
  Longest length: 1214348 bp
  Contig bases in this tier: 4016795 bp
  Q20 bases in this tier: 3985915 bp
  Q20 base percentage: 99.2%
  Top tier N50 supercontig length: 660143 bp
  Top tier N50 supercontig number: 3

Middle tier (4000000 bp -- 4200000 bp): 
  Supercontig number: 2
  Average length: 131224 bp
  Longest length: 131657 bp
  Contig bases in this tier: 262447 bp
  Q20 bases in this tier: 261539 bp
  Q20 base percentage: 99.6%
  Middle tier N50 supercontig length: 131657 bp
  Middle tier N50 supercontig number: 1

Bottom tier (4200000 bp -- end): 
  Supercontig number: 116
  Average length: 1491 bp
  Longest length: 13698 bp
  Contig bases in this tier: 172942 bp
  Q20 bases in this tier: 157876 bp
  Q20 base percentage: 91.2%
  Bottom tier N50 supercontig length: 2333 bp
  Bottom tier N50 supercontig number: 7



*** Constraints ***
No. of satisfied constraints in contigs:        8666
No. of unsatisfied in distance in contigs:      6425
No. of satisfied links in scaffolds:            1949
No. of unsatisfied in dist. in scaffolds:         83
No. of unsatisfied due to singlets:             3765
No. of unsatisfied due to short scaffolds:       573
No. of unsatisfied due to scaffold ends:         136
No. of other unsatisfied constraints:           1282
No. of redundant constraints:                   1515
Total no. of satisfied constraints:            10615
Total no. of unsatisfied constraints:          12264
Total no. of constraints:                      24394


*** Genome Contents ***
Total GC count: 2522563, (56.7%)
Total AT count: 1929621, (43.3%)
Total NX count: 0, (0%)
Total: 4452184


*** Fosmid Coverage ***
total fosmids used: 8384
total length of these fosmids: 7110019 bp
Estimated fosmid coverage (total fosmids Q20 bases over genome): 1.19X