Publication

A vertebrate case study of the quality of assemblies derived from next-generation sequences.

Genome Biol. 2011 Mar 31;12(3):R31. [Epub ahead of print]

Abstract

The unparalleled efficiency of next-generation sequencing (NGS) has prompted widespread adoption, but significant problems remain in the use of NGS data for whole genome assembly. We explore the advantages and disadvantages of chicken genome assemblies generated using a variety of sequencing and assembly methodologies. NGS assemblies are equivalent in some ways to a Sanger-based assembly yet deficient in others. Nonetheless, these assemblies are sufficient for the identification of the majority of genes and can reveal novel sequences when compared to existing assembly references.

Authors

Ye L, Hillier LW, Minx P, Thane N, Locke D, Martin JC, Chen L, Mitreva M, Miller JR, Haub KV, Dooling D, Mardis ER, Wilson RK, Weinstock GM, Warren WC.