Publication

Conservation of Ebp-type Pilus Genes among Enterococci and Demonstration of Their Role in Adherence of Enterococcus faecalis to Human Platelets.

Infect Immun. 2011 Jul;79(7):2911-20. Epub 2011 Apr 18.

Abstract

Ebp are endocarditis and biofilm associated pili of Enterococcus faecalis that are also important in experimental UTIs. Our analyses, using available genomes, found that the ebp locus is unique to enterococci. In E. faecalis, the ebp locus is very highly conserved and only 1/473 E. faecalis isolates tested lacked ebpABC while only 1.2% had the bee pilus locus. No other pilus-encoding operon was identified in 55 available genomes indicating that the vast majority of E. faecalis (unlike Enterococcus faecium and streptococci) have a single pilus locus. Surface expression studies showed that Ebp pili were produced in vitro by 91/91 BHI+serum grown E. faecalis isolates and that strain OG1RF expressed pili at even higher levels in rat endocarditis vegetations. However, Ebp expression was restricted to 30-72% of E. faecalis cells, consistent with a bistability mode of expression. We also evaluated E. faecalis interactions with human platelets and found that growth of E. faecalis in BHI+serum significantly enhanced adherence to human platelets and that sortase deletion mutants (?srtA, ?bps, and ?bps?srtA) were markedly defective. Further studies identified that Ebp pili, but not the MSCRAMMs Ace or Fss2, mediate adherence of E. faecalis to platelets. Taken together, our data show that the immunogenic (in human endocarditis patients) and commonly expressed Ebp pili, which are known to be important for experimental endocarditis, are highly conserved and mediate adherence to platelets, suggesting that Ebp pili may be a reasonable immunotherapeutic target for prevention or possibly treatment of endocarditis caused by this species.

Authors

Nallapareddy SR, Sillanpää J, Mitchell J, Singh KV, Chowdhury SA, Weinstock GM, Sullam PM, Murray BE.

Institute Authors