Decoding tumor genomes reveals clues to the spread of a deadly form of breast cancer that affects younger women and African-Americans.
Massively parallel DNA sequencing technologies provide an unprecedented ability to screen entire genomes for genetic changes associated with tumour progression. Here we describe the genomic analyses of four DNA samples from an African-American patient with basal-like breast cancer: peripheral blood, the primary tumour, a brain metastasis and a xenograft derived from the primary tumour. The metastasis contained two de novo mutations and a large deletion not present in the primary tumour, and was significantly enriched for 20 shared mutations. The xenograft retained all primary tumour mutations and displayed a mutation enrichment pattern that resembled the metastasis. Two overlapping large deletions, encompassing CTNNA1, were present in all three tumour samples. The differential mutation frequencies and structural variation patterns in metastasis and xenograft compared with the primary tumour indicate that secondary tumours may arise from a minority of cells within the primary tumour. Supplementary information is available here.
Following are supplementary tables related to the manuscript titled "Genome remodelling in a basal-like breast cancer metastasis and xenograft." 15 April 2010, Nature.
The documents are available in Excel format.
Last update: 15 April 2010
Timothy Ley, M.D., Tammi Vickery, Robert Fulton, Richard K. Wilson, Ph.D., Mike McLellan, Michelle O'Laughlin, Michael Wendl, Ph.D., Lucinda Fulton, Liz Appelbaum, Lisa Cook, Li Ding, Ph.D., Lei Chen, Ph.D., Kelley , Joshua McMichael, Joelle Veizer, Heather Schmidt, Elaine R. Mardis, Ph.D., Dong Shen, Ph.D., David Larson, Ph.D., Aaron P Graubert, Craig Pohl, Catrina Fronick, Amy Hawkins, Jim Eldred, Jerry Reed, Feiyu Du