Large-Scale Sequencing and Analytical Processing of ESTs.

Methods Mol Biol. 2009;533:1-35.


Expressed sequence tags (ESTs) have proven to be one of the most rapid and cost-effective routes to gene discovery for eukaryotic genomes. Furthermore, their multipurpose uses, such as in probe design for microarrays, determining alternative splicing, verifying open reading frames, and confirming exon/intron and gene boundaries, to name a few, further justify their inclusion in many genomic characterization projects.


Mitreva M, Mardis ER.

Institute Authors

Makedonka Mitreva, Ph.D.