The molecular characterization of parasitic nematodes and development of novel control strategies can benefit from genomic approaches. The high-throughput generation of expressed sequence tags (ESTs) from numerous nematode cDNA libraries is now providing thousands of new gene sequences, and their availability in public databases will facilitate broad characterization of their function. A project at Washington University's Genome Sequencing Center is nearing the completion of 235,000 5' ESTs from 28 nematode species (227,272 ESTs as of October 2003). Another 32,078 ESTs from seven species have been contributed by a sister project at the Sanger Institute and Edinburgh University. Sequences are immediately submitted to the database of expressed sequence tags (dbEST) at GenBank and are also available from specialized Web sites (Table 1A). Nematode ESTs have diverse applications (e.g., Blaxter et al., 2002; Jasmer et al., 2001; Murray et al., 2001; Pleasance et al., 2003; Scholl et al., 2003; Srinivasan et al., 2002), and strategies for their use have been reviewed (Blaxter et al., 1999; Grant and Viney, 2001; Marra et al., 1998; McCarter et al., 2000; Parkinson et al., 2001; Parkinson et al., 2003).