PolyBayes nucleotide alignment evaluation


This page is provided to demonstrate the the way PolyBayes evaluates a slice (column) of a multiple sequence alignment. To use the alignment evaluation facility, please specify the multiply aligned nucleotides (up to a depth of 20), together with the associated (PHRAP or PHRED) base quality values. You do not need to fill in all available positions. Non-used positions, indicated by '-', will be omitted during evaluation. You may also want to change the prior values provided under 'Parameter settings'. Changing any of the parameters will change the calculated SNP probabilty score. After specifying the alignment slice and the parameters, activate the evaluation code by clicking on the 'Evaluate' button. Results will be displayed at the bottom of the page. You can now modify the input and re-evaluate your data.


Input


Nucleotide alignment specification
Sequence Nucleotide Base quality
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
Parameter settings
Description Value
Expected (prior) polymorphism rate
Expected transition/transversion ratio