Library Construction

10X Genomics
Single-cell gene expression sequencing and analysis
The Chromium System enables researchers to discover previously inaccessible genomic information at massive rate and scale, including: phase resolved structural variants, single nucleotide variants and indels, as well as dynamic gene expression profiles from tens of thousands of individual cells.

Illumina
- Nextera Mate Pair Libraries
- 3Kb-8Kb
- cDNA
- TruSeq Stranded Total RNA (strand specific) – for human, mouse & rat only (due to ribodepletion step)
- TruSeq Stranded mRNA (strand specific) – any organism

Roche
Small Insert Fragment Libraries (200-700bp)
- Manual
- KAPA Hyper, KAPA LTP, IDT Low input
- Automated
- KAPA HTP, KAPA Hyper PCR-free