As the expense of next-generation sequencing (NGS) has plummeted, whole-genome sequencing (WGS) has become increasingly attractive due to the large quantity of genetic information it reveals. However, challenges in obtaining sufficiently deep reads and in managing the extraordinary volume of data remain. Target-enrichment approaches—capturing specific subsets of the genome via hybridization with probes and subsequent isolation and sequencing—in conjunction with NGS offer attractive, less costly alternatives to WGS. In applications requiring greater sequencing depth than is practical with WGS, such as whole-exome sequencing (WES) and customized or disease-specific sequencing panels, this type of targeted sequencing approach focuses analysis on the most relevant genomic regions, although it runs the risk of missing crucial variants outside the targeted regions. In this webinar, we investigate the advantages and disadvantages of the multiple sequencing modalities available today, from WGS to WES to targeted sequencing. Choosing which approach to use depends largely on the scientific question being asked.
During this webinar the speakers will:
- Discuss the relative pros and cons of WGS, WES, and targeted sequencing
- Highlight specific situations in which each application offers the best solution for their particular sequencing needs
- Answer viewer questions during the live broadcast.
Speaker: Bob Fulton, M.S.Washington University School of Medicine
St. Louis, MO
Speaker: Shawn Levy, Ph.DDiscovery Life Sciences
Moderator: Jackie Oberst, Ph.D.Science/AAAS