Expected Results

Gene Expression
Limit of Detection:
•  0.5fM spike-in control (~1 copy per cell); 90% of the time
Fold-change Sensitivity:
•  >1.5 fold (>5 copies per cell)
•  >2 fold change (>1 copy per cell)
miRNA Assay
Limit of Detection:
•  ≤ 0.5fM (~10 copies per cell)
Fold-change Sensitivity:
• > 2 fold change
Copy Number Variation
Reproducibility:
•  99%
Number of Copies Detected:
•  0-4, multiallelic
miRGE Assays
Limits of Detection:
•  For miRNA: >2.5 copies per cell copy per cell
•  For mRNA: 1 copy per cell
Fold-change Sensitivity:
•  >2 fold change